体内外消化高直链抗性水稻淀粉的波谱特性

摘要

In this study, the spectroscopic properties of in vivo and in vitro digestive resistant starches from a high-amylose transgenic rice line （TRS） established through antisense RNA inhibition of starch branching enzymes were investigated by X-ray powder diffraction and Fourier transform infrared spectroscopy. The results showed that the amylose contents of non-digestive TRS resistant starch significantly increased. TRS starch was C-type crystalline starch, a mixture of A- and B-type crystalline starches. The degradation of the A-type crystalline starch was faster than that of B-type starch, and the B-type crystalline starch showed a high resistance to digestion. The amorphous starch of starch granule was easy to be degraded. These data could be useful for various applications of high-amylose crop in the food and nonfood industry.%以基因工程培育出来的高直链抗性淀粉水稻（TRS）为材料，利用粉末X射线衍射、傅里叶变换红外光谱分析技术，研究体内外消化TRS淀粉的波谱特性。结果表明：不被消化的抗性淀粉直链淀粉含量显著升高；TRS淀粉为C型晶体淀粉，由A型和B型晶体淀粉组成，其中A型晶体比B型晶体消化降解快，B型晶体具有抗消化降解的作用；TRS淀粉的无定形区域易被消化降解。