沙拉沙星分子印迹聚合物的制备及其性能研究

摘要

以沙拉沙星为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用本体聚合制备沙拉沙星的分子印迹聚合物。通过振荡吸附实验对模板分子和功能单体的比例进行优化。印迹聚合物和空白聚合物的等温吸附线表明,印迹聚合物形成的孔穴对沙拉沙星的吸附量高于空白聚合物。在吸附过程中模板分子沙拉沙星与印迹聚合物形成两种结合位点,两种结合位点的解离常数分别为0.321μg/mL和1.577μg/mL,对沙拉沙星最大表观吸附量分别为1.249mg/g和3.222mg/g。吸附动力学实验结果显示聚合物对沙拉沙星的吸附在7h达到平衡,选择性试验表明印迹聚合物对沙拉沙星具有较好的吸附特性。%A molecularly imprinted polymer （MIP） was synthesized by bulk polymerization method using propionamide as the template, methylacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. Adsorption experiments were carried out on the MIP with perfect molar ratio of 1：26 between the template and the monomer. The adsorption isothermal curves of MIP and NMIP showed that the adsorption quantity of sarafloxacin in the cavity formed by MIP was higher than that of NMIP. Two kinds of binding sites were observed between the imprinted polymer and the template （sarafloxacin）. Their deassiocation constants （Kd） were 0.321 μg/mL and 1.577 μg/mL, and the apparent maximum absorption capacity （Qmax） were 1.249 mg/g and 3.222 mg/g, respectively. The adsorption dynamic curve of MIP displayed that the adsorption of sarafloxacin on MIP could reach equilibrium within 7 h. Therefore, MIP has good adsorption capacity for sarafloxacin.