基于磁性免疫传感器的牛乳中四环素残留检测方法的构建

摘要

A highly sensitive and rapid immunofluorescence assay for the detection of tetracycline residues in raw milk based on magnetic beads was developed. The limit of detection (LOD) and limit of quantiifcation (LOQ) of this method were 0.03 and 0.20 ng/mL, respectively. The lfuorescence intensity was linear with the concentration of tetracycline in the range of 0.25 to 32 ng/mL with a coefifcient of determination of 0.995 9 (R2= 0.995 9). The recoveries of TC spiked at different concentrations were between 82.6% and 97.0%, indicating that the method is accurate. Furthermore, precision, calculated as relative standard deviation (RSD), was below 5.0% for intra-day and inter-day determinations, indicating good repeatability of the method. The results of tetracycline residues in raw milk determined by this method and HPLC-UV were highly consistent.%建立一种高灵敏磁性光学免疫传感检测方法，可用于生鲜乳中四环素残留的快速检测。方法的检出限为0.03 ng/mL，定量限为0.20 ng/mL；四环素质量浓度在0.25～32 ng/mL时，荧光信号值与质量浓度之间具有良好的线性关系，其回归方程决定系数（R2）为0.9959；本方法准确度高，四环素不同添加水平条件下的回收率在82.6%～97.0%之间；该方法具有良好的重复性，测定结果的日内精密度和日间精密度均在5.0%以内；本方法与高效液相色谱-串联紫外检测结果高度吻合。