不同温度对β-葡聚糖酶分批发酵动力学的影响

摘要

The effect of different temperatures on the growth ofBacillus subtilisZJF-15, substrate consumption andβ-glucanase production was studied by conducting batch fermentation experiments in a 5-L fermentation tank. The fermentation conditions were determined as 400 r/min, 1.0 L/(L·min), 7.0, 5%, 0.6 and 35–37℃forrotating speed, ventilation rate, initial fermentation pH, inoculum quantity, medium loading coefficient and culture temperature, respectively. The results showed that increased culture temperature led to a higher reaction rate, accelerated bacterial growth and metabolism, earlier production ofβ-glucanase, faster enzyme inactivation, shortened fermentation period and finally reduced production ofβ-glucanase. The kinetic equations for bacterial growth, substrate consumption andβ-glucanase production of Bacillus subtilis ZJF-15 were established by batch fermentation.%在5 L发酵罐中研究不同温度对枯草芽孢杆菌（Bacillus subtilis）ZJF-15菌体生长、底物消耗和产酶量的影响。分批发酵的工艺条件为：搅拌转速400 r/min、通气量1.0 L/（L·min）、pH 7.0、接种量5%、装液系数0.6、发酵温度35～37℃。结果表明：温度升高会导致反应速率加大、生长代谢加快、生产期提前、酶失活速率加快、发酵周期缩短、最终产酶量减少。实验建立了B. subtilisZJF-15分批发酵的菌体生长、底物消耗和β-葡聚糖酶产酶的动力学方程。