UPLC和Q-Exactive高分辨质谱仪联用鉴定大黄鱼中的甘油三酯分子组成

摘要

应用超高效液相色谱和电喷雾/四极杆-静电场轨道阱的质谱联用技术,分析大黄鱼脂肪中甘油三酯的组成.采用Hypersil Gold C18柱(150 mm×4.6 mm,2.1 μm),流动相为82％甲醇(含0.1％甲酸)溶液和18％异丙醇溶液,混合后添加0.05％乙酸铵溶液,以等度洗脱方式分离大黄鱼脂肪中的甘油三酯组分,柱后流出液采用电喷雾离子源,以正离子模式采集一级质谱分子离子和二级质谱的碎片离子信息,然后与Lipidblast数据库中甘油三酯信息进行比对.研究结果显示,超高效液相色谱和Q-Exaetive高分辨质谱仪联用技术快速、准确鉴定出大黄鱼脂质中含有28种甘油三酯.PoOL、PoSO、PPO、PPoO、PSO、MPO、POL、PoSA 8种甘油三酯占总量的87.87％以上,其中PoOL (C16∶1、C18∶1、C18∶2)相对含量最丰富,占35.261％.%The triglyceride composition of Pseudosciaena crocea lipids was analyzed by ultra performance liquid chromatography-electrospray ionization/quadrupole/electrostatic field orbit trap high resolution mass spectrometry (UPLC-ESI/Orbitrap-MS).The chromatographic separation was performed on a Hypersil Gold C18 column (150 mm × 4.6 rm,2.1 μm) using a mobile phase consisting of methanol (including 0.1％ formic acid)-isopropyl alcohol (82:18,V/V) and 0.05％ ammonium acetate.The analytes were detected by ESI/Orbitrap-MS in the positive ion mode to obtain mass and tandem mass spectra together and then the results obtained were compared with those from the Lipidblast database.A total of 28 triacylglycerols were identified by UPLC-ESI/Orbitrap-MS).Among these,PoOL was the most abundant (35.261％),and PoOL,PoSO,PPO,PPoO,PSO,MPO,POL and PoSA together accounted for 87.87％ of the total triacylglycerols.