COMPREHENSIVE METABOLITE IDENTIFICATION OF DM4, A MAYTANSINOID ANTI-TUMOR AGENT, IN S100 FRACTIONS OF HUMAN LIVER MICROSOMES, BY UPLC-QTOF MASS SPECTROMETRY

摘要

Maytansinoids are highly cytotoxic molecules; several of them are investigated in cancer therapy, as conjugates with antibodies (ADCs) targeting specific cells. This study describes the identification of the metabolites of the maytansinoid DM4, after incubation with S100 fractions of human liver microsomes (HLM) [1]. Particularly, we wanted to gain better understanding of truly metabolic processes from chemical degradation of the molecule.