A new method was developed for the determination of nuciferine in Nelumbinis Folium and liensinine in Nelumbinis Plumula by capillary electrophoresis coupled with electrochemilumolinescence (ECL) detection.The optimized experimental conditions were determined as follows:Ru(bpy)32+ concentration,5 mmol/L;detection potential,1.20 V;10 mmol/L phosphate buffer (pH 5.74) as running buffer,60 mmol/L (pH 8.30) phosphate buffer contained in the detection reservoir;injection time,10 s;separation voltage,13 kV.Detection limits (LOD) of 7.7 x 10-7 mol/L (RSN =3) for nuciferine and 7.8 × 10-7 mol/L (RsN =3) for liensinine were obtained.Relative standard derivations of electrophoretic peak area and migration time were 3.76% and 0.83% for 6.8 × 10-5 mol/L nuciferine and 4.28% and 1.37% for 3.1 x 10-5 mol/L liensinine from five replicate determinations,respectively.The method can be applied to detect nuciferine in lotus leaves and liensinine in Nelumbinis Plumula with satisfactory results.%建立毛细管电泳-电化学发光法同时测定荷叶中荷叶碱与莲子心中莲心碱含量的方法.对三联吡啶钌(Ru(bpy)32+)溶液浓度、检测电位、磷酸盐缓冲液浓度和pH值、进样时间和分离电压等实验条件进行考察和优化.结果表明,在检测电位为1.20 v,运行缓冲液为10 mmol/L磷酸盐缓冲液(pH 5.74),5 mmol/L Ru(bpy)32+溶液,检测池内磷酸盐缓冲液浓度为60 mmol/L (pH 8.30),进样电压为8 kV,进样时间为10s,分离电压为13 kV,荷叶碱检出限为7.7×10-7 mol/L (RSN=3),莲心碱检出限为7.8×10-7 mol/L (RsN=3).对浓度为6.8×10-5 mol/L荷叶碱和3.1×10-5 mol/L莲心碱的标准品溶液进行5次平行测定表明:荷叶碱峰面积的相对标准偏差(relative standard deviation,RSD)为3.76%,迁移时间RSD为0.83%;莲心碱峰面积RSD为4.28%,迁移时间RSD为1.37%.该方法可用于测定荷叶中的荷叶碱与莲子心中莲心碱含量.
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