响应面试验优化复合酶法提取碎米荠多糖工艺及其抗氧化活性

摘要

利用人工种植的碎米荠为原料,研究其粗多糖的提取工艺参数及抗氧化活性.首先对提取条件的单因素进行优化,在单因素试验基础上,进行提取条件的响应面优化.单因素优化条件为:质量分数2％复合酶(m(纤维素酶):m(果胶酶)=2:1)、酶解时间90 min、酶解温度60℃、酶解pH 4.0.响应面优化结果为:酶解时间91.8 min、酶解温度57.1℃、酶解pH 4.17.在此条件下,碎米荠粗多糖提取率最高,粗多糖提取率预测值为4.14％,验证实验得到实际粗多糖的平均提取率为4.07％;与理论预测值相比,其相对误差约为1.62％.抗氧化活性研究结果显示,碎米荠多糖具有抗氧化活性,且效果优于VC.该实验结果为碎米荠多糖的提取以及多糖的性质研究提供理论依据.%The extraction of crude polysaccharides from cultivated Cardamine hupingshanensis was optimized by the combined use of one-factor-at-a-time method and response surface methodology.The results of one-factor-at-a-time experiments demonstrated hydrolysis with a mixture (2:1,m/m) of cellulase and pectinase at a dosage of 2％ (m/m) at 60 ℃ and an initial pH of 4.0 for 90 min to be optimum for the extraction ofpolysaccharides.Furthermore,the extraction conditions op tmized by response surface methodology were as follows:hydrolysis time,91.8 min;hydrolysis temperature,57.1 ℃;and intial pH,4.17.Under these conditions,the predicted maximum yield of polysaccharides of 4.14％ was obtained,and experiments gave an average value of 4.07％,indicating a relative error of about 1.62％ the predicted and experimental values.The results of antioxidant assays showed that Cardamine hupingshanensis.polysaccharides possessed antioxidant activity better than that of VC.Our experimental results provide an experimental basis for studying the extraction and properties of Cardamine hupingshanensis polysaccharides.