为探究温度对鱼源腐败希瓦氏菌碳源利用的影响,利用多孔平板技术获得5、15、25℃和33℃条件下其生长动态,采用修正的Gompertz方程,构建动力学模型,获取动力学参数,结合孔平均颜色变化率及利用面积,探究其碳源利用效果.结果表明,25℃时最大比生长速率(μmax)最大,迟滞期(λ)最小,总体碳源利用能力和活性最强,15℃时次之,5℃时活性降至约25℃的1/6,33℃时最弱;5～25℃范围内,温度和√μ、√1/λ面呈现良好的线性关系.5～33℃时糖类和羧酸类利用率较高,比例分别为30％、29％.糖类(糊精、D-麦芽糖、α-D-葡萄糖、D-阿拉伯醇、水苏糖)、羧酸类(L-苹果酸、L-乳酸、乙酰乙酸)、氨基酸类(L-丝氨酸、氨基乙酰-L-脯氨酸、L-天冬氨酸和L-谷氨酸)和胺/酰胺类、脂肪酸/脂类和其他类中(明胶、丙酮酸甲酯、吐温40、L-组胺)利用较好.通过对腐败希瓦氏菌碳源利用分析,通过对腐败希瓦氏菌碳源利用和动力学分析,可为深入其代谢活性与水产品营养相关性等提供理论依据.%In order to investigate the carbon source utilization profile of Shewanella putrefaciens at different temperatures,a kinetic model describing the growth of S.putrefaciens in multi-well plates at 5,15,25 and 33 ℃ was developed using modified Gompertz equation and the kinetic parameters maximum specific growth rate (μmax) and lag phase duration (λ) were obtained.Moreover,based on average well color development (AWCD) and utilization area,the carbon utilization rate of S.putrefaciens was analyzed.The results showed that S.putrefaciens presented the highest μmax and lowest λ at 25 ℃.Moreover,at this temperature,the highest carbon utilization and cell viability were observed followed by growth at 15 ℃;the cell viability at 5 ℃ was one-sixth of that at 25 ℃,and the lowest cell viability was found at 33 ℃.Temperature in the range of 5-25 ℃ exhibited a good linear relationship with √μ and √1/λ.The utilization rates of of carbohydrates and carboxylic acids were 30％ and 29％ at 5-33 ℃,respectively.Carbohydrates (dextrin,D-maltose,α-D-glucose,D-arabinol and stachyose),carboxylic acids (L-malic acid,L-lactic acid and acetoacetic acid),amino acids (L-serine,amino-acetylL-proline,L-aspartic acid and L-glutamic acid),fatty acids/lipids,amines/amides and other substances (gelatin,methyl pyruvate,Tween 40 and L-histamine) could be better utilized by the strain.
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