响应面分析法优化槐米总黄酮的提取工艺

摘要

The effects of volume, time, temperature, the ratio of solid-fluid (g:mL), on the yield of total flavone were studied and the parameters for optimum yield were obtained by using RAS software. The results showed that the optimum parameters was: 60 percent ethanol volume, 2.2 h,1:17.9 (g: mL),temperature80.0℃. In this situation, the extraction yield of total flavone is 18.46 %.%通过对槐米总黄酮提取,考察体积分数、提取时间、提取温度、料液比四因素对槐米总黄酮提取率的影响.用RAS软件程序对试验数据进行二次响应面分析,对3项工艺参数进行优化,得出槐米总黄酮提取的二次回归方程;通过分析得出槐米总黄酮提取的最佳条件为:60%乙醇(体积分数),提取2.2 h,料液比(g:mL)1:17.9,温度80.0℃.在此条件下测得的总黄酮提取率为18.46%.