西番莲黄酮的提取及抗氧化活性研究

摘要

To research better methods to extract flavonoids from passion fruit, and to study the anti-oxidative ability of flavonoids. Dry and shatter passion fruits, extracted flavonoids using ethanol, and measure the elimination effects of the flavonoids on hydroxyl radicals (·OH) and superoxide anion radical (O2-·). Results show that when the ethanol concentration is 70%, the liquid ratio (g/mL) is 1∶30, the temperature is 80℃, and extraction time is 2 hours, the flavonoid extraction yield is at an ideal level, which is 1.73%. The elimination effects of the flavonoids on ·OH and O2-·increase with increment of the concentration of flavonoids, and the effects are better than that of VC at the same concentration level. Passion fruit's flavonoids have generally good anti-oxidative ability.%研究从西番莲中提取黄酮的较佳方法，并对其抗氧化活性进行研究。将西番莲烘干粉碎，用乙醇提取黄酮，并测定提取的西番莲黄酮对羟自由基（·OH）、对超氧阴离子自由基（O2-·）清除作用。结果表明，当乙醇浓度为70%、料液比（g/mL）为1∶30、提取温度为80℃、提取时间为2 h时提取效果较好，黄酮得率为1.73%。西番莲黄酮对·OH和O2-·的清除率随黄酮浓度的增大而升高，比同浓度的VC清除率高。西番莲黄酮有较好的抗氧化活性。