树莓与蓝莓中多酚类成分的HPLC测定

摘要

To assay the quantity of activities from blue berry and raspberry by absorption spectrometry and HPLC. The content of Gallic acid, protocatechuic acid, catechin, epicatechin, procyanidin B2, cyanidin, ellagic acid, raspberry ketone, resveratrol and salicylic acid were determined by HPLC analysis was detected by chromatogra-phy on Kromasil C18 (4.6 mm×150 mm) chromatographic column. The mobile phase was chromatographic purity acetonitrile (A)-water solution containing 0.1%phosphoric acid(B) gradient elution, 0-16 min, 5 %A;19 min, 10 %A;23 min, 13 %A;40 min, 14 %A;43 min, 17 %A;48 min, 22 %A;51 min, 24 %A;52 min, 24 %A;58 min, 26%A;60 min, 30 %A;65 min, 60%A;68 min, 70%A;72 min-78 min, 100 %A, flow rate was 1 mL/min atλ280 nm. The content of phenolic compounds in these three kinds of berry was different .The content of ellagic acid from high to low order:black raspberry (0.97μg/g), red raspberry (0.51μg/g), blue berry (0.36μg/g). Blue berry and raspberry are particularly rich in phenolic compounds, which were the basics of their biological active diversity.%利用HPLC法对树莓与蓝莓中的生理活性成分进行含量测定。HPLC法采用Kromasil C18色谱柱（150 mm，4.6 mm）；流动相采用色谱纯乙腈（A）-含0.1%磷酸水溶液（B）梯度洗脱，0~16 min，5%A；19 min，10%A；23 min，13%A；40 min，14%A；43 min，17%A；48 min，22%A；51 min，24%A；52 min，24%A；58 min，26%A；60 min，30%A；65 min，60%A；68 min，70%A；72 min~78 min，100%A；流速为1 mL/min；柱温30℃；检测波长为280 nm；每次进样10μL，测定树莓与蓝莓95%乙醇提取物中没食子酸、原儿茶酸、儿茶素、表儿茶素、原花青素B2、矢车菊素、鞣花酸、树莓酮、白藜芦醇、水杨酸的含量；这十种多酚类成分在三种浆果中含量高低不等，其中鞣花酸含量在黑树莓中最高（0.97μg/g），红树莓次之（0.51μg/g），蓝莓最低（0.36μg/g）。蓝莓与树莓中富含多种多酚类物质，是其具有多种生物活性的物质基础。