超临界提取生姜挥发油的抗氧化及抑菌活性

摘要

研究超临界CO2提取的生姜挥发油的体外抗氧化活性以及抑菌活性，选用DPPH自由基清除率、超氧阴离子自由基、羟自由基清除率和还原力4种指标来评价生姜挥发油的抗氧化活性。数据显示超临界CO2提取得到的生姜挥发油具有良好的抗氧化活性，具有清除DPPH·、O2-·、羟自由基活性及还原性。在抑菌试验中，超临界CO2提取生姜挥发油对试验所用供试菌种都表现了一定的抑菌活性，其对青霉的抑制能力较强，MIC和MBC均为0.78%。其次对黑曲霉的抑制作用也较好，MIC为0.78%，MBC为3.13%，与细菌相比，生姜挥发油对真菌的抑制能力更强。%The antioxidant and antimicrobial activities of volatile oil from ginger by supercritical CO 2 extraction were studied. Evaluation of the antioxidant effect of volatile oil from ginger was focused primarily on scavenging of 2,2-Diphenyl-1-picril hydrazyl radical(DPPH·), superoxide negative ion radical(O2-·) and hydroxyl radi-cal as well as reducing capacity. The data showed the volatile oil of ginger had an antioxidant activity , activities of eliminating DPPH·, O2-·, hydroxyl radical and reducing capacity. Under the conducted experiments the volatile oil from ginger by supercritical CO2 extraction also demonstrated a good antimicrobial activity to the mi-crobial strain tested, penicillium was the most vulnerable to the volatile oil from ginger (MIC, MBC 0.78%), followed by aspergillus niger(MIC 0.78%, MBC 3.13%). Compared with bacteria, volatile oil from ginger had a higher inhibiting ability to fungus.