A rapid and effective method was established for the determination of trace imidaeloprid pesticide residue in fruit and vegetable drink by ultra performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). After the sample was extracted by 0.1%acetic acid-acetonitrile, it concentrated and puri-fied by amino solid phase extraction column. The separation of targeted compound was performed on a Waters Atalantis T3 chromatographic column(2.1 mm×150 mm, 3μm) using 0.1%formic acid-acetonitrile as mobile phase under gradient elution mode. The linear range of imidaeloprid was in the range of 0.5 ng/mL-50.0 ng/mL with a correlation coefficient of 0.999. The detection limit was 0.15μg/kg and the quantitative limit was 0.5μg/kg. The recovery rate was 93.2%-96.9%.%建立一种快速、 有效的超高效液相色谱-串联质谱法测定果蔬饮料中痕量吡虫啉农药残留的方法.样品经0.1%醋酸-乙腈提取后,采用氨基固相萃取小柱进行净化和富集.采用Waters Atalantis T3色谱柱(2.1 mm×150 mm,3μm)分离,以0.1%甲酸-乙腈为流动相的梯度洗脱模式下,吡虫啉标准在0.5 ng/mL~50.0 ng/mL浓度范围内线性良好,相关系数r2为0.999,检出限为0.15μg/kg,定量限为0.50μg/kg,加标回收率达到93.2%~96.9%.
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