A HPLC-PDA method was established for determination of trace Sudan Ⅲ in foodstuffs. The chro-matographic conditions were:Symmetry C18 column (4.6 mm ×250 mm, 5μm) was used with mobile phases composed of acetonitrile-acetone (80-20):water=95:5, under the detection wavelength for 504 nm, 1.0 mL/min as the flow rate, column temperature 15℃. The peak area and the concentration of Sudan Ⅲ showed a good lin-ear relationship:A=26310ρ-911.78(ρ:μg/mL), R2=0.9992, in the range of 0.03μg/mL-101.86μg/mL. The above method precision was good and stability was strong. The adding standard recoveries of four kinds of sam-ples were between 97.33 %-100.00 %, according with the requirement of the determination. HPLC-PDA method was accurate and fast and it could be used for the determination of Sudan Ⅲ. To further validate the ex-istence of Sudan Ⅲ in these samples by mass spectrometry. Mass spectrometry showed that Sudan Ⅲ molecular ion peak was m/z 353, main cracked into m/z 156 and m/z 197.%建立了利用高效液相色谱(HPLC)-二极管阵列检测器(PDA)法测定食品中苏丹红Ⅲ的方法.采用的色谱条件为:Symmetry C18色谱柱(4.6 mm×250 mm,5μm),用乙腈-丙酮(80-20):水=95:5作为流动相,检测波长为504 nm,流动相流速为1.0 mL/min,柱温为15℃.利用色谱检测苏丹红Ⅲ的浓度在0.03μg/mL~101.86μg/mL时,组分的峰面积与浓度之间线性关系良好,苏丹红Ⅲ的线性方程为A=26310ρ-911.78(ρ:μg/mL),R2=0.9992.该方法精密度良好,稳定性较强.检测的4种样品的加标回收率在97.33%~100.00%之间,符合测定要求.高效液相色谱法,方法准确,快速,适用于苏丹红Ⅲ的测定.通过质谱,进一步验证了食品中苏丹红Ⅲ的存在.利用质谱检测到,苏丹红Ⅲ的分子离子峰为m/z 353,主要碎裂成m/z 156和m/z 197.
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