To optimize the ultrasonic-assisted extraction of proantho-cyanidins of litchi pericarp and the contents of flavan-3-ol of were studied from litchi pericarp. Box-Behnken design (BBD) was applied to evaluate the effects of four independent variables(the smash degree, the ratio of solvent to material, ultrasonic duration and ultrasonic power ) on the absorbance values of proanthocyanidins of litchi pericarp. Under the optimized conditions, the contents of flavan-3-ol of were analyzed by the methods of catalytic colorimetry with ferric i-ons and HPLC analysis from litchi pericarp. The results showed that the optimal conditions were as follows: the smash degree of litchi pericarp was 71. 91 mesh, the ratio of solvent to material was 29. 97 : 1 (V : m) , ultrasonic duration was 21. 75 min, ultrasonic power wasrn390. 48 W with the ultrasonic extraction method. Under the above conditions, the confirmed tests absorbance value of proanthocyanidins was 2. 157 Abs, which was close relation with the predicted absorbance value of proanthocyanidins was 2. 189 86 Abs. The contents of flavan-3-ol showed that procyanidins was 89. 32 mg/g, gallic acid was 1. 11 mg/g, epigallocatechin was 87. 09 mg/g, catechin was 3. 37 mg/g, epicatechin was 31. 15 mg/g and epicatechin-gallate was 7. 02 mg/g by the methods of catalytic colorimetry with ferric ions and HPI-C analysis.%优化荔枝皮中原花青素超声波辅助提取工艺,并对其黄烷-3-醇类成份进行分析定量.用Box-Behnken Design设计考察荔枝皮粉碎度、料液比、超声时间和超声波功率4个因素对荔枝皮中原花青素提取的影响程度.在最佳工艺参数条件下,超声破碎荔枝皮1次,采用铁盐催化比色法和HPLC法分析得出荔枝皮黄烷-3-醇类成份及含量.荔枝皮中原花青素超声波辅助提取最佳工艺参数为荔枝皮粉碎度71.91目、料液比1∶29.97(m ∶V)、超声时间21.75 min和超声波功率390.48W.在最佳提取工艺参数下超声提取荔枝皮中原花青素1次,其吸光度值为2.157 Abs,与原花青素吸光度预测值2.189 86 Abs接近.通过铁盐催化比色法HPLC法分析并得出荔枝皮黄烷-3-醇类成份及含量:原花青素、没食子酸、表没食子儿茶素、儿茶素、表儿茶素和表儿茶素没食子酸酯分别为89.32,1.11,87.09,3.37,31.15,7.02 mg/g.
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