基于纳米金与适配体的赭曲霉毒素A检测方法研究

摘要

A high sensitivity fluorescence detection method for ochratoxin A (OTA) based on OTA aptamers and gold nanoparticle (AuNPs) was established,which employed OTA aptamers as recognition element and AuNPs to quench the fluorescence emission from fluorescein.The results indicated that the detection limit is 10 nmol/L and a linear relationship between the fluorescent intensity ratio and the logarithm of OTA concentration was plotted in the range of 25 ～1 000 nmol/L (R2=0.991).The detection method showed high fea sibility and reliability.Compared with traditional method,this method greatly improved the detection efficiency and provided techni cal support for more effective identification of OTA.%以赭曲霉毒素A适配体作为识别分子荧光素发射的荧光作为检测信号、纳米金作为荧光信号淬灭剂,建立一种高灵敏度的赭曲霉毒素A荧光检测方法.结果表明:该检测体系检测限达到10 nmol/L,在25～1 000 nmol/L浓度范围具有线性关系,相关系数为0.991,具有较好的实用性.该荧光检测法同传统方法相比,提高了检测效率,为更好地进行赭曲霉毒素A检测控制提供了技术支持.