In this study, the electrochemical cyclic voltammetry method was developed for the determination of activity of glucose oxidase.The ferrocene methanol was introducing as the redox probes on cyclic voltammetry, using glassy carbon electrode as working electrode, saturated calomel electrode as reference electrode, platinum wire electrode as the counter electrode and the peak current value of different enzyme activity was required.There was a linear relationship between the peak current in enzyme concentration of 1.282~2.377 U/mL with the correlation coefficient as 0.998, the detection limit as 1.282 U/mL.The enzyme activity of spores from dormancy to germination was detected by this new method, as the enzyme activity of spores decreased after increasing first, reaching the maximum (2.130 U/mL).The traditional methods were tested at the same time.Compared with the traditional method, the proposed electrochemical method has the characteristics of accurate, fast and convenient.%建立了电化学循环伏安法测定葡萄糖氧化酶活性的方法.该方法引入二茂铁甲醇作为氧化还原探针,以玻碳电极作为工作电极、饱和甘汞电极作为参比电极、铂丝电极作为辅助电极,采用循环伏安法,得到不同酶活的峰电流值,在酶浓度为0.128~2.377 U/mL,酶浓度与峰电流之间存在线性关系,相关系数为0.998,检测下限为0.128 U/mL.运用该法测定了孢子萌发过程中酶活的变化,随着孢子的萌发,酶活先增加,达到最大值(2.130 U/mL)后再次下降,同时采用传统滴定法进行对照.结果表明,相较于传统方法,电化学方法具有准确、快速和简便的特点.
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