玉米黄色素提取工艺优化及其抗癌活性研究

摘要

探究超声辅助酶法制备玉米黄色素的最佳工艺,以及玉米黄色素对人胃腺癌细胞(BGC-823)生长的抑制作用.通过单因素和响应面试验,探讨超声辅助酶法制备玉米黄色素的最佳条件;用制备的玉米黄色素处理BGC-823细胞,并进行形态学观察和MTT法细胞存活率检测,初步考察其抗癌活性.超声辅助酶法提取玉米黄色素的最佳条件为:酶活力10 000 U/g,超声功率400 W,酶解温度35℃,该条件下提取率达最大,为(72.64士0.42)μg/g;细胞形态学观察及MTT细胞活力检测显示玉米黄色素对BGC-823细胞具有一定的生长抑制作用.超声辅助酶法提取玉米黄色素是一种有效的提取方法,制备的玉米黄色素对BGC-823细胞生长有较强的抑制作用.%Objective:To study the best preparation technology of maize yellow pigment by ultrasonic assisted with enzymatic method,and the inhibitory effect of them on the growth of human gastric cancer cells (BGC-823).Methods:The optimum conditions of maize yellow pigment were extracted by ultrasonic assisted with enzymatic method by single factor and response surface experiments.The inhibition of maize yellow pigment on BGC-823 cells was detected by morphological observation and MTT assay to evaluate the antitumor activity.Results:the maize yellow pigment best extraction condition of ultrasonic with enzymatic method is:enzyme activity 10 000 U/g,ultrasonic power 400 W,enzymolysis temperature 35 ℃,the maximum extraction yield was (72.64 ± 0.42) μg/g under the condition,the maize yellow pigment has a certain inhibitory effect on BGC-823cell were studied by cell morphological observation and MTT test.Conclusion:The extraction of maize yellow pigment by ultrasonic assisted with enzymatic method is a kind of effective extraction method,the maize yellow pigment prepared has strong inhibitory effect on BGC-823 cells.