黄蜀葵花黄酮抗氧化性及对DNA氧化损伤的保护作用

摘要

采用石油醚、乙酸乙酯和正丁醇对黄蜀葵花乙醇提取物进行分级萃取,得到石油醚相、乙酸乙酯相、正丁醇相和水相4个不同极性成分.采用DPPH·清除、·OH清除和H2O2清除试验考察了其抗氧化活性,研究了正丁醇相对·OH和H2O2诱导DNA氧化损伤的保护作用,并利用HPLC-MS对正丁醇中黄酮类物质进行了鉴定.结果表明:黄蜀葵花黄酮主要富集于乙酸乙酯相和正丁醇相;乙酸乙酯相对DPPH·的清除能力最好,IC50值为0.038 6 mg/mL,而正丁醇相对·OH和H2O2的清除能力较好,IC50值分别为0.225 0,0.575 7 mg/mL;正丁醇相对·OH和H 2O2诱导的DNA氧化损伤都有保护作用;正丁醇相中含有11种黄酮类化合物.%The ethanol extracts of flowers from Abelmoschus manihot was extracted by petroleum ether、ethyl acetate and n-butyl alcohol,and four different polar portions were obtained.The scavenging effect of different phases on DPPH ·,· OH and H2O2 were studied to research the antioxidant activities of each phase.Moreover,the protection of n-butyl alcohol phase on DNA oxidative damage was also studied,and the flavonoids component was identified by HPLC-MS.The results showed that the ethyl acetate phase had the best scavenging effect on DPPH ·,with IC50value 0.038 6 mg/mL.However,the n-butyl alcohol phase had scavenge · OH and H2 O2 best,and the ICs0 values were 0.225 0 and 0.575 7 mg/mL,respectively.The n-butyl alcohol phase was proved to show protective effect in some degree on DNA oxidative damage.,and totally 11 kinds of flavonoids were found in the n-butyl alcohol phase.