本研究采用响应面法优化超声波提取鸡枞菌多糖的工艺条件,并进一步研究了其抗氧化活性.在单因素的基础上,选取超声波作用时间、温度及液固比为影响因子,应用Box-Behnken中心组合进行3因素3水平的实验设计,以多糖提取率为响应值,进行响应面分析.结果表明,超声波提取鸡枞菌多糖的最佳条件为超声波作用时间36min、温度41℃、液固比37:1时,可得最大多糖提取率,预测值为6.36%,与验证值(6.32%)基本一致.鸡枞菌多糖对羟基自由基(·OH)、超氧阴离子自由基(O2-·)及DPPH自由基都有较强的清除能力,具有良好的抗氧化活性.%Response surface analysis methodology was used for optimizing the ultrasound extraction process of Colly-bia albuminosa polysaccharides. Using extraction rate of Collybia albuminosa polysaccharides as the indicator, effects of extraction temperature, time and water-material ratio extraction on the extraction rate of polysaccharide were in-vestigated. Based on single factor studies, a three-variable, three-level Box-Benhnken experimental design and re-sponse surface methodology were used to obtain the optimized extraction conditions of polysaccharides. The optimum ultrasound extraction conditions for Collybia albuminosa polysaccharides were extraction time of 36min, extraction temperature of 41℃, and water-material ratio of 37:1 (mL/g), under which the extraction rate of polysaccharide was up to 6.36%, and the actual extraction rate is 6.32%. Moreover, the scavenging effects of Collybia albuminosa polysaccharides on ·OH, O2-· and DPPH radical was strong, the results showed that the Collybia albuminosa polysaccharides had good antioxidant activity.
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