蛹虫草液态发酵过程中有效成分的动态积累变化

摘要

The dynamic variation of the accumulation of biomass, Cordyceps polysaccharides, cordycepic acid and cordycepin were compared through fermentation process. The results showed that 70% of Cordyceps polysaccharide, Cordyceps acid and cordycepin was distributed in the fermentation broth. The optimum fermentation period was 12d by considering these three products. The results of 10 L fermentation tank submerged culture test showed that the biomass reached 24.5 mg/mL, which increased 19.86% compared with shaking flask culture, while the Cordyceps acid, CP, cordycepin content were 7.43 mg/mL, 2.82 mg/mL, 90.73 μg/mL respectively, which increased 8.3% , 13.7% and 15.6% compared with shaking flask culture.%通过对蛹虫草4号菌株进行摇瓶液态发酵培养，考察了蛹虫草发酵过程中发酵液及菌丝体生物量、虫草多糖、虫草酸及虫草素含量的动态积累变化情况。结果表明：70％以上的虫草多糖、虫草酸、虫草素分布在发酵液中。蛹虫草菌在第10天生物转化量达到最大值20．44mg／mL，虫草酸、虫草多糖、虫草素含量分别在第11、13、14天达到最大值，综合考虑3种产物的最佳发酵周期，将蛹虫草发酵时间定为12d。10L发酵罐深层培养试验的结果表明，生物量达24．5mg／mL，比摇瓶培养提高19．86％，而虫草酸、虫草多糖、虫草素含量分别为7．43、2．82、90．73μg／mL，比摇瓶培养分别提高8．3％、13．7％和15．6％。