鉴定金黄色葡萄球菌的多重PCR方法

摘要

To establish a rapid multiplex PCR assay to identify and differentiate Staphylococcus aureus The genes sea, sec, sed, see, mecA and nuc were grouped into a multiplex PCR reaction system. By optimizing the multiplex PCR primers, reaction condition and cycle parameters the muhiplex PCR can identify and differentiate Staphylococcus aureus rapidly and accurately. The established multiplex PCR assay was applied in this study to identify 47 strains of Staphylococcus aureus. The results showed that the genetypes were 100% consistent with those single PCR amplification. The multiplex PCR assay in this study can be used to identify the Staphylococcus aureus in a single reaction. This assay is an effective method for identifying taphylococcus aureus isolated from foods.%建立了一种快速鉴定和鉴别金黄色葡萄球菌的多重PCR方法。利用金黄色葡萄球菌的特异基因、耐甲氧西林基因和4种肠毒素基因nnc、mecA和sea、sec、sed、see建立6重PCR反应体系，并对PCR体系，引物浓度进行优化。6对PCR引物均能特异地扩出相应的目的基因。对47株金黄色葡萄球菌的检测结果与应用单重PCR鉴定结果完全一致。实验所建立的多重PCR方法能在一个反应体系中鉴定和鉴别金黄色葡萄球菌，为食品中金黄色葡萄球菌的快速检测提供了一种有效的检测手段。