采用毛细管电泳法分析荸荠中的黄酮类化合物,测定了芦丁、槲皮素、山奈酚和绿原酸的含量。研究了缓冲液的种类、离子浓度、pH值、分离电压和运行温度等一系列电泳参数,得出优化的电泳条件为：缓冲液为20mmol/L磷酸二氢钾-20 mmol/L硼砂（pH 7.8）,紫外检测波长202 nm,分离电压24 kV,运行温度30℃。在此条件下,上述4种物质在7 min内即可分离,并在3.125～100 mg/L内有良好的线性关系,相关系数R2均在0.999以上,相对标准偏差（RSD）：迁移时间为0.78%～0.98%;峰面积为6.5%～8.6%。在5,10,20 mg/kg的添加水平下,4种物质的加标回收率为80%～98%。该方法简便、快速、重现性好,可推广应用于植物中4种黄酮类物质的同时检测。%Capillary electrophoresis (CE) method has been developed for the determination of flavonoids and the content of rutin, quereetin, kaempferol, Chlorogenic acid in water chestnut. Based on the effects of composition and concentration of running buffer, pH, separation temperature and separation voltage, the optimal conditions were found as: the running buffer consisted of 20 mmol/L KHzP04-20 mmol/L borax, the UV detection wavelength was 202 nm, separation voltage was 24 kV, separation temperature was 30℃. Under these optimal chromatographic conditions, the four flavonoids can be separated within 7 minute. Moreover, the calibration curves of the concentrations of four fla- vonoids and their corresponding peak areas showed good linearity in the range 3. 125 from 100 mg/L. The correlation coefficient was above 0. 999. The RSD of migration times was between O. 78% and 0.98% , and the RSD of peak are- as was between 6.5% and 8.6 %. At the spiked concentration of 5.0,10.0,20.0 mg/kg, the average recoveries of the four flavonoids ranged from 80% to 98%. This method is very simple, rapid, good repeatability and can be ap- plied to the determination of four flavonoids in plants.
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