离子色谱法测定蜂花粉与蜂王浆中的葡萄糖、果糖、蔗糖和麦芽糖

摘要

An ion chromatography method was developed for determination of sugars in bee pollen and royal jel- ly. Sugars were extracted with hot water and cleaned up by solid phase extraction after protein precipitation by aceto- nitrile. Sugars were separated on a Carbora PAl0 column with NaOH/NaOAc solution as mobile phase. The limit de- tection of glucose, fructose, sucrose and maltose were 0. 12,0. 14,0.21,0.33 rag/L, respectively. The relative standard deviations of bee pollen were 4.4% - 6.3% and the recoveries are 93.5% - 102.2% , the relative standard deviations of royal jelly were 6.7% - 10.2% and the recoveries were 95.0% - 103.5%. The method was applied for the analysis of 20 bee pollen samples and 20 royal jelly samples. The average concentrations of glucose, fructose, su- crose and maltose in bee pollen samples were 9.9 - 20.6% , 14.2 - 26.9% , 1.8% - 8.3% , 0.33% - 1.4% , re- spectively. The average concentrations of glucose, fructose, sucrose and maltose in royal jelly samples were 6.2 - 8.3% ,7.0 - 8.7% ,0.38% ~ 3.6% , 0.27% - 0.83% , respectively. The method is simple and suitable for a rou- tine quality sugar control of bee pollen and royal jelly.%研究了蜂花粉与蜂王浆中葡萄糖、果糖、蔗糖和麦芽糖的离子色谱分析方法。样品用热水溶解,乙腈沉降蛋白,使用2个固相萃取小柱去除色素与脂肪等干扰成分后,以CarboPac PA10分析柱为色谱柱,氢氧化钠和乙酸钠溶液为流动相进行离子色谱分离。葡萄糖、果糖、蔗糖和麦芽糖的检出限分别为0.12,0.14,0.21,0.33mg/L。蜂花粉的回收率93.5%～102.2%,精密度为4.4%～6.3%,蜂王浆的回收率为95.0%～103.5%,精密度为6.7%～10.2%。利用该方法对20个蜂花粉和20个蜂王浆样品进行了分析,花粉中葡萄糖含量为9.9%～20.6%,果糖14.2%～26.9%,蔗糖1.8%～8.3%,麦芽糖0.33%～1.4%;王浆中葡萄糖含量为6.2%～8.3%,果糖7.0%～8.7%,蔗糖0.38%～3.6%,麦芽糖0.27%～0.83%。该方法简单、灵敏度高,可作为标准方法对蜂花粉与蜂王浆中葡萄糖、果糖、蔗糖和麦芽糖进行分析。