采用三硝基苯磺酸法测定乳基婴幼儿配方奶粉中的有效赖氨酸

摘要

A method of determination of available lysine in infant formula powder with 2,4,6-trinitrobenzene sulfonic acid（TNBS） was established.The ether usage,hydrolysis time and incubation time with TNBS were studied.Good linearity was obtained in the range of 0.02~0.12 mg/mL（r2=0.999 3）;the determination limit was 3.72 μg/mL;and quantitative limit was 10.23 μg/mL and the sample spiked recoveries were in range of 86%~115% with the reproducibility RSD of 1.55%~5.57%.%采用三硝基苯磺酸法建立了乳基婴幼儿配方奶粉中有效赖氨酸的测定方法,研究了乙醚用量、水解时间以及三硝基苯磺酸的孵化时间对水解液吸光度的影响。方法线性范围为0.02～0.12 mg/mL,相关系数r2=0.999 3,检出限为3.72μg/mL,定量限为10.23μg/mL,样品重复性相对标准偏差RSD为1.55%～5.57%,回收率为86%～115%。