乌饭树叶提取物抗氧化活性研究及组分分析

摘要

采用乙醇浸提、大孔树脂纯化制备乌饭树叶提取物,考察其在不同体系中的抗氧化活性,并对提取物的主要成分进行HPLC分析.结果表明:乌饭树叶提取物具有较高的抗氧化能力,其清除DPPH自由基能力与Vc相当,总抗氧化能力、还原力、抗脂质过氧化能力和清除羟自由基能力与待测物浓度存在明显的量效关系.HPLC结果表明,乌饭树叶提取物中主要存在异槲皮苷、槲皮素和山奈酚等3种黄酮类化合物,其含量分别为267.8,61.6和98.0mg/g.%In this paper, the vaccinium bracteatum leaf extract was prepared by ethanol extraction and purified by macroreticular resin.The antioxidant activity of Vaccinium bracteatum leaf extract in different systems were studied and analyzed by HPLC.The results showed that the Vaccinium bracteatum leaf extract had high antioxidant activity, and equivalent to Vc in terms of scavenging DPPH free radical capacity.In addition, the total antioxidant capacity, reducing power, anti-lipid peroxidation ability, free radical scavenging ability were in dose-effect relationship.HPLC analysis results indicated the major components of the Vaccinium bracteatum leaf extract were flavonoids compounds including isoquercitrin (267.8 mg/g), quercetin (61.6 mg/g), and kaempferol (98.0 mg/g).