Eco‑friendly synthesis of silver nanoparticles using leaf extract of Flemingia wightiana: spectral characterization, antioxidant and anticancer activity studies

摘要

In the present investigation, we report simple, robust and eco-friendly synthesis of silver nanoparticles (AgNPs) using leafextract of Flemingia wightiana. The leaf extract of F. wightiana acts as reducing agent which reduces the silver ions intoFW-AgNPs, as well as stabilizing agent by capping around them. Biosynthesized FW-AgNPs were characterized by differentspectroscopic techniques. UV–Vis spectrum showed the characteristic SPR peak between 380 and 460 nm. EDX analysisrevealed the presence of metallic silver at 3 keV. XRD analysis clearly revealed that FW-AgNPs are crystalline in naturewith FCC structure. TEM analysis depicted the spherical morphology with 20–40 nm in size. DLS analysis showed thataverage hydrodynamic size and PDI value of FW-AgNPs were found to be 47.6 nm and 4.5 respectively. BiosynthesizedFW-AgNPs showed high negative zeta potential value of − 25.3 mV. FTIR analysis revealed the participation of polyphenolsand proteins in the bioreduction and stabilization of FW-AgNPs. FW-AgNPs showed strong DPPH and H_2O_2scavengingactivity with IC50 values of 71.96 and 80.59 μg/mL respectively. Further FW-AgNPs also showed effective cytotoxicityagainst cancer cells including SKOV3 and COLO205 with maximum inhibition of 83.2% and 75.9% respectively.