碳源对烟曲霉WHSW-01产壳聚糖酶的诱导条件优化

摘要

In order to improve the production of chitosanase from Aspergillusfumigates WHSW-01,effects of different carbon sources on chitosanase production was studied.Aspergillus fumigatus WHSW-01 chitosanase was considered as an inducible enzyme.Results indicated that chitosan hydrozate was the optimal inducer for the production of chitosanase from Aspergillus fumigatus WHSW-01.The optimal addition amount of chitosan hydrozate was 40％ and the optimal addition time of it was after the fermentation continued 12h.Finally,6.78U/mL of chitosanase was obtained when Aspergillus fumigates WHSW-01 was cultured under these conditions.%为提高烟曲霉WHSW-01产壳聚糖酶的能力,首先确定了该菌产壳聚糖酶受碳源的诱导作用.通过在以壳聚糖为基础碳源的培养基中添加不同碳源,考察该菌细胞生长及产酶的情况,确定了壳聚糖水解液对烟曲霉WHSW-01产壳聚糖酶具有最好的诱导作用.通过考察壳聚糖水解液添加量及添加时间对烟曲霉WHSW-01产壳聚糖酶的影响,探讨了壳聚糖水解液对烟曲霉WHSW-01产壳聚糖酶的最佳诱导条件:发酵12h后添加发酵液总体积40％的壳聚糖水解液对烟曲霉产壳聚糖酶进行诱导,有利于壳聚糖酶的产生,在此条件下烟曲霉发酵产酶高峰期壳聚糖酶酶活为6.78 U/mL.