QuEChERS-高效液相色谱法检测红葡萄酒中多菌灵和甲霜灵杀菌剂残留

摘要

A method was developed for the determination of benzimidazole and amides pesticides in red wine by QuEChERS-high performance liquid chromatography (QuEChERS-HPLC).In this article,extraction salts and purification reagents of QuEChERS were optimized reasonably,according to the nature of benzimidazole and amides pesticides.After comparison,NaCl and NaAc were chosen as extraction salt and PSA as purification reagent finally.The calibration curve of carbendazim and metalaxyl showed good linearity at the range 0.2 ～0.5mg/L,with the correlation coefficient more than 0.999.Recoveries at three spiked concentration levels ranged from 85.3％ to 98.34％,with the relative standard deviation (RSD) at the range from 3.61％ to 10.22％.The method provides higher universality and extraction efficiency due to its simple pre-treatment and less extraction solvent compared with other conventional method.So it is a good method to analyze the residues of benzimidazole and amides pesticides in red wine.%建立了以QuEChERS作为前处理方法,采用高效液相色谱(HPLC)检测红葡萄酒中多菌灵和甲霜灵的有效方法.结合考虑2种农药的性质,对QuEChERS的提取盐和净化试剂进行优化,最终选用NaCl与NaAc为提取盐,PSA作为吸附净化剂.多菌灵和甲霜灵均在0.2～5.0mg/L范围内具有良好的线性相关性,线性系数在0.999以上.多菌灵和甲霜灵的加标回收率为85.3％～98.34％,相对标准偏差(RSD)值为3.61％～10.22％.QuEChERS方法与其他常规前处理方法相比,通用性强、操作简便、成本低、提取效率高,可以作为检测红葡萄酒中该类农药残留的有效前处理方法.