改良QuEChERS-高效液相色谱法检测柑橘中的多菌灵

摘要

建立了柑橘中多菌灵残留量的改良QuEChERS-高效液相色谱快速检测方法．样品用10％醋酸乙腈溶液提取、醋酸钠和无水硫酸镁盐析后，取上清液，加入N-丙基乙二胺（PSA）与石墨化炭黑（GCB）吸附剂净化，用高效液相色谱-UV检测器测定，外标法定量．多菌灵在0．05～5．0mg/L（r＝0．9983）范围内与峰面积呈良好线性关系；方法检出限为0．01 mg/kg；回收率为72．0％～99．0％；相对标准偏差小于4％．该方法操作简便、准确、快速、成本较低，适用于柑橘中多菌灵残留量的测定．%A developed method for determination of carbendazim in citrus by QuEChERS-HPLC was established. Samples were extracted with acetonitrile containing 10% acetic acid and salted out by anhydrous MgSO4 and CH3 COONa. Purifying by N-propyl ethylenediamine (PSA)cartridge and graphitized carbon black(GCB),the carbendazim in the solution was determined by HPLC-UV. The linear range is 0. 05-5. 0mg/L (r=0. 9983)and the detection limit was 0. 01 mg/kg. The recovery was from 72% to 99%,relative standard deviation was less than 4%. The proposed method was simple,accurate,quick,low-cost and ap-plicable for the determination of carbendazim residue in Citrus.