蜜环菌液态发酵培养基及补料分批发酵工艺的优化

摘要

Through optimizing the Armillaria mellea liquid fermentation medium and 2 m3 fermentation tank production process,the optimum medium for Armillaria mellea was medium containing corn flour of 60 g/L,soybean powder of 20 g/L,ethanol of 3 mL/L,VB1 of 0.03 g/L,KH2PO4 of 1.5 g/L,MgSO4 · 7H2O of 0.75 g/L,α-amylase of 0.1 g/L with the initial pH 3.0.The best breeding time for next step fermentation was 6 d.After fermentation in 50 L fermentor for 8 d,the biomass could reach 26.80 g/L.After fermentation in 200 L fermentor for 6 d,the biomass was 15.35 g/L.Fermentation can be moved to the next step at this point,2 m3 fermentor with supplementary fermentation.After fermentation for 8 d,the biomass could reach 22.65 g/L.%通过对蜜环菌液态发酵培养基和2 m3发酵罐生产工艺的优化,得到蜜环菌适宜的培养条件为:玉米粉60 g/L,豆粕20 g/L,无水乙醇3 mL/L,VB10.03 g/L,KH2PO41.5 g/L,MgSO4· 7H2O 0.75 g/L,α-淀粉酶0.1g/L,初始pH3.0,当摇瓶种子培养到第6天,菌丝量19.24 g/L时,移种到50 L发酵罐,培养8d,其菌丝量可达26.80 g/L.200 L种子发酵罐培养6d,其菌丝量为15.35 g/L,此时可转入下一级培养,2 m3发酵罐发酵采用补料发酵的方式,培养8d,其菌丝量可达22.65 g/L.