Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Polysaccharide Production by Tricholoma Mongolicum Imai

摘要

The fermentation medium of Tricholoma Mongolicum Imai for producing intracellular polysaccharide and extracellular polysaccharide was optimized by response surface methodology. The effects of carbon source, nitrogen source and mineral salt on polysaccharide production were examined by single factor experiments, glucose and yeast was found to be the best carbon source and nitrogen source. Based on this, the optimization of the Box-Benhnken design combined with response surface analysis showed that the optimal medium contained 40g/L glucose; 20g/L yeast extract; 0.40% KH_2PO_4; 0.40% MgSO_4.The intracellular polysaccharide production and extracellular polysaccharide in medium reached 2.0512g/L and 2.394 g/L, respectively.