TRICHOLOMA MATSUTAKE STRAINS, CULTURING METHOD THEREOF AND PRODUCTION OF TRICHOLOMA MATSUTAKE USING CULTURE MEDIUM THEREOF

摘要

PURPOSE: A method of production of Tricholoma matsutake by the following process consisting of separation of Tricholoma matsutake SJM, multistage liquid culture of the Tricholoma matsutake SJM and then the inoculation thereof around the circumference of a pine tree in a flushing method is provided. It permits a mass production of Tricholoma matsutake without the addition of equipment. CONSTITUTION: Tricholoma matsutake SJM(KCCM 10458) is inoculating into a solid medium containing 10g/L glucose, 2g/L yeast extract, 1g/L KH2PO4, 5g/L Ebioze, 20g/L agar and distilled water and then cultured for 30 to 40 days at 25deg.C. The cultured Tricholoma matsutake SJM is inoculated into a culture medium containing 20g/L glucose, 1.5g/L yeast extract, 1.5g/L bean flour and distilled water and then cultured for 30 days at 25deg.C. Thereafter, the culture liquid is inoculated into a culture medium containing 20g/L yellow sugar, 1.5g/L bean flour, 3ml/L corn oil and distilled water and then cultured for 30 days at 20 to 25deg.C while blowing sterile air. The culture liquid is inoculated into a culture medium containing 200g/L yellow sugar, 3g/L bean flour, 3ml/L corn oil and distilled water and then cultured for 30 days at 20 to 25deg.C while blowing sterile air.