Preliminary Analysis of Genetic Characteristics of Edible Fungus Tricholoma matsutake Isolated from Yajiang, China, by AFLP Technique

摘要

A total of Tricholoma matsutake strains isolated from Yajiang County,Sichuan Province of China were analyzed by using AFLP technique.The results showed that the genetic characteristics of Tricholoma matsutake strains can be properly revealed by AFLP,and among the 10 Tricholoma matsutake strains,the genetic aspects were very similar.At the level of 90% similarity,all the strains collected together,and at the similarity of 92%,three AFLP groups formed.Simultaneously,the effects of quantity of the primers on the second AFLP PCR were determined.The optimal quantity of Eco+1/Mse+1,Eco+2/Mse+2 and Eco+3/Mse+3 primer pairs used in the AFLP was 1 pmol/5 pmol,0.6pmol/10pmol and 0.1pmol/8pmol,respectively.