文摘
英文文摘
DECLARATION
CERTIFICATION
DEDICATION
ACKNOWLEDGEMENTS
UNITSANDABBREVIATIONS
Resume
CHAPTER 1 LITERATURE REVIEW
1.1. Rice blast disease
1.1.1. Introduction
1.1.2 The causal organism
1.2 The perfect stage of the rice blast fungus
1.2.1 Mating types and fertility in Magnaporthe grisea
1.2.2 Mating type occurrence in China
1.3 Pathotype diversity in Magnaporthe grisea population
1.3.1. Pathotypes diversity of the rice blast fungus in china
1.4 Population genetic of rice blast fungus
1.4.1 Population variation and evolution
1.4.2 Population genetic analysis of Magnaporthe grisea
1.4.3 Population genetic diversity of the blast fungus in China
1.4.4 Factors influencing the fungal genetic diversity
1.4.5 Molecular genetic tools used to study fungal populations
1.5 Objectives
REFERENCES
CHAPTER 2 DISTRIBUTION OF MATING TYPE AND IDENTIFICATION OF PATHOTYPES IN MA GNAPORTHE GRISEA ISOLATES COLLECTED FROM BLAST NURSERIES IN HUNAN PROVINCE, CHINA
2.1 Introduction
2.2 Materials and methods
2.2.1 Collection
2.2.2 Isolation and storage of the blast fungus
2.2.3 Standard isolates for mating types determination
2.2.4 Crossing method for mating types determination
2.2.5 Asci and ascospores formation
2.2.6 Pathogenicity assay and determination of the pathotype of each isolate
2.2.7 Coding of the pathotypes
2.3 Results
2.3.1 Isolation of the blast fungus Magnaporthe grisea
2.3.2 Distribution of the mating types and female isolates of Magnaporthe grisea in different collection sites
2.3.3 Crosses among fertile isolates of M. grisea from Hunan.province
2.3.4 Chinese race identification of isolates of Magnaporthe grisea from Hunan province
2.3.5 Relation between fertility and Chinese race of Magnaporthe grisea
Discussion
2.4.1 Diversity of the mating types of Magnaporthe grisea in blast nurseries in Hunan province
2.4.2 Diversity of the Chinese races of Magnaporthe grisea in blast nurseries in Hunan province
REFERENCES
CHAPTER 3 POPULATION GENETIC DIVERSITY OF THE RICE BLAST FUNGUS MAGNAPORTHE GRISEA IN BLAST NURSERIES OF HUNAN PROVINCE
3.1 Introduction
3.2 Materials and methods
3.2.1 Isolates of Magnaporthe grisea
3.2.2 Culture medium
3.2.3 Preparation of the fungal mycelium
3.2.4 Composition and preparation of buffers solutions
3.2.5 DNA extraction
3.2.6 Selection of RAPD primers and amplification reaction
3.2.7 Electrophoresis of the amplified products
3.2.8 Clustering analysis
3.3 RESULTS AND DISCUSSION
3.3.1 Results
3.3.2 Discussion
REFERENCES
CHAPTER 4 Population Genetic Diversity of Fertile Magnaporthe grisea Isolates from Blast Nurseries in Hunan Province
4.1 Introduction
4.2 Materials and methods
4.2.1 Fungi strains
4.2.2 Extraction of the genomic DNA
4.2.3 Scars primers
4.2.4 Scars-PCR amplification conditions
4.2.5 Analysis of SCAR molecular data and construction of the dendrogram
4.3 Results
4.3.1 Scars amplification
4.3.2 Lineage structure of fertile Magnaporthe grisea isolates
4.3.3 Discussion
References
CHAPTER 5 GENERAL CONCLUSION
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