液相色谱串联质谱法测定白菜中的甲醛含量

摘要

In this paper,formaldehyde-2,4-dinitrophenylhydrazone in Chinese cabbage was produced by formaldehyde through 2,4-dinitrophenylhydrazine derivatives methods.Liquid chromatography tandem mass spectrometry analysis results showed that the detection limit of formaldehyde in this method was 3.06 μg/kg.When the spiking amount in Chinese cabbage was 1-18mg/Kg,the formaldehyde amount was linearly correlated to peak area (r2 =0.994).When the amount of formaldehyde was 0.5,1,5 mg/kg,recoveries were between 102％ to 105％,and the relative standard deviations were between 3％ and 8％ with good stability and precision.%文中使用2,4-二硝基苯肼衍生法使白菜中甲醛生成甲醛-2,4-二硝基苯腙,再使用乙腈提取富集甲醛-2,4-二硝基苯腙(衍生物),后经液相色谱串联质谱对其浓度进行检测.结果显示,该法中甲醛的检出限为3.06 μg/kg,当白菜中甲醛添加含量在1～ 18 mg/kg时,甲醛线性良好(r2=0.994).对白菜中3种浓度(0.5、1、5 mg/kg)甲醛添加进行6次平行回收实验,结果表明,3种浓度平行添加回收率在102％～105％,相对标准偏差为3％ ～8％,回收稳定,精密度良好.