小麦低聚肽和谷氨酰胺对大鼠胃肠黏膜损伤的保护作用

摘要

Purpose:The present study was aimed to investigate the effect of wheat oligopeptide and glutamine on non-steroidal anti-inflammatory drugs (NSAIDs) which induced gastrointestinal mucosa damage in rats.Method:70 SD rats were randomly divided into control group,damage group,low,medium and high doses of wheat peptide group (the doses were 20,100 and 500 mg/(kg · d),wheat gluten group and glutamine group (the doses were both 20 mg/(kg · d)).The rats were administered once a day for 30 days.Before sacrificing,NSAIDs were infused into the digestive tract twice.Serum,stomach and small intestine mucosa samples were collected to observe the changes in serum cytokine,pathological section of stomach and small intestine,as well as enzyme activity of small intestinal antioxidase and opioid receptor mRNA expression.Results:Wheat oligopeptides administration reduced gastrointestinal mucosa damage,and significantly decreased the level of TNF-alpha in serum.Oxidative stress was significantly increased after NSAID infusion..Wheat oligopeptides increased GSH-Px activity in mucous membrane of small intestine.Mu opioid receptor mRNA expression decreased more significantly in wheat oligopeptides treated rats than in the control group.Conclusion:Non-steroidal anti-inflammatory drugs can induce small intestinal damage in rats and wheat oligopeptides administration can protect gastrointestinal tissue against NSAID-induced gastrointestinal damage and oxidative stress.Lower doses of wheat peptide group showed the strongest effects,even better than glutamine group.%观察灌胃小麦低聚肽和谷氨酰胺对非甾体类药物(NSAIDs)致大鼠胃肠黏膜损伤的保护作用.挑选70只SD大鼠随机分为空白对照组、损伤组及低、中、高剂量小麦低聚肽组[灌胃剂量分别为20、100、500 mg/(kg·d)]和小麦蛋白组、谷氨酰胺组[灌胃剂量均为20 mg/(kg· d)].每天灌胃1次,连续灌胃30 d.大鼠处死前,用非甾体类药物灌胃损伤大鼠胃肠2次.取大鼠血清、胃和小肠黏膜组织,观察血清细胞因子、胃肠病理切片、小肠黏膜抗氧化酶和阿片受体mRNA表达的变化.结果显示非甾体类药物可以显著增加小肠黏膜的氧化应激水平,小麦低聚肽能上调小肠黏膜中GSH-Px的活力,具有一定的抗氧化功能,能够减轻胃肠黏膜损伤.小麦低聚肽还可以显著降低了血清中TNF-α含量,下调mu-阿片受体mRNA的表达.说明非甾体类药物可以诱导大鼠胃肠黏膜损伤的模型,小麦低聚肽可以有效减少大鼠胃肠黏膜损伤.低剂量小麦低聚肽组作用最强,效果优于谷氨酰胺组.