水解小麦蛋白肽对大鼠急性酒精性胃黏膜损伤的保护作用

摘要

Objective:The present work was aimed to examine the protective effect of hydrolyzed wheat protein peptide (HWP) in the model rats with acute gastric mucosal damage induced by ethanol. Methods: Hydrolyzed wheat protein peptide (167, 333 or 667 mg/(kg·d) (in body mass), cimetidine (65 mg/(kg·d)), or distilled water were administered daily by gavage for 30 days before the rats were treated with anhydrous ethanol (1 mL) and then euthanized after 1 h. Histological observation was performed to evaluate the antioxidant and anti-inflammatory activity of HWP as well as its impact on serum physiological and biochemical parameters and the expression of caspase-3 and epidermal growth factor receptor (EGFR). Results: Histological observation showed that ethanol-induced gastric mucosal damage was attenuated by hydrolyzed wheat protein peptide pretreatment. Hydrolyzed wheat protein peptide remarkably increased the superoxide dismutase (SOD) activity, epidermal growth factor (EGF) and prostaglandin E-2 (PGE2) levels in serum. Furthermore, it dramatically decreased malondialdehyde (MDA), platelet-activating factor (PAF) and interleukin-8 (IL-8) levels in serum. The expression level of caspase-3 in stomach was significantly decreased in hydrolyzed wheat protein peptide-treated rats when compared with the model group;however, the expression level of EGFR was markedly increased. Conclusion:Ethanol-induced gastric mucosal damage in rats can be protected against by hydrolyzed wheat protein peptide. The potential mechanism may be associated with the enhancement of antioxidant, anti-inflammatory and anti-apoptotic functions.%目的：研究水解小麦蛋白肽对大鼠急性酒精性损伤模型胃黏膜损伤的保护作用。方法：将60只Wistar大鼠随机分成空白对照组、胃黏膜损伤模型对照组、水解小麦蛋白肽低、中、高剂量组（剂量分别为167、333、667 mg/（kg·d）（以体质量计，下同））、阳性对照组（甲氰咪胍组65 mg/（kg·d）），每组各10只。采用酒精灌胃致胃黏膜损伤，给予水解小麦蛋白肽30 d后，观察胃黏膜组织的大体变化和病理组织学改变，测定大鼠血清丙二醛（malondialdehyde，MDA）、血小板活化因子（platelet-activating factor，PAF）、表皮生长因子（epidermal growth factor，EGF）、前列腺素E2（prostaglandin E-2，PGE2）、白细胞介素8（interleukin-8，IL-8）含量及超氧化物歧化酶（superoxide dismutase，SOD）活力，大鼠胃组织半胱氨酸天冬氨酸蛋白酶3（caspase 3）、表皮生长因子受体（epidermal growth factor receptor，EGFR）表达水平。结果：水解小麦蛋白肽能明显改善胃黏膜组织损伤，降低损伤积分（P＜0.05），提高损伤抑制率；各剂量组均能提高大鼠血清PGE2含量、EGF含量、SOD活力及胃组织EGFR表达水平，降低大鼠血清MDA含量、IL-8含量及胃组织Caspase 3表达水平（P＜0.05），低、中剂量组能降低血清PAF含量（P＜0.05）。结论：水解小麦蛋白肽对大鼠急性酒精性胃黏膜损伤具有一定保护作用，其机制可能与增强胃黏膜保护因子、提高抗氧化、抗炎及抗凋亡等作用有关。