微波辅助提取沙田柚囊衣中柚皮苷及其对抗氧化性的影响

摘要

采用微波提取技术对沙田柚囊衣中柚皮苷的提取工艺进行优化,并对提取液的抗氧化活性进行分析.以单因素试验为基础,选取溶剂体积分数、微波功率和料液比3个因素,根据Box-Behnken Design中心组合设计原理进行响应面分析,建立柚皮苷得率的二次多项数学模型.得到提取柚皮苷的最佳工艺条件:提取溶剂为体积分数52％乙醇,微波功率587 W,料液比1∶20 (g∶ mL),柚皮苷的提取量为15.581 0 mg/g.此外,根据DPPH法、FRAP法及ABTS法3种方法测定的提取液的抗氧化性表明,有效的微波处理能显著影响提取物的抗氧化性.%Microwave-assisted extraction of naringin from Shatian pomelo white layer using was optimized by response surface methodology(RSM.The antioxidant activity of extracts were analyzed in vitro.On the basis of the single-factor experiment results,use extracting volume ratio,microwave power and solid-to-solvent ratio as the indexes,based on central composite experimental design of the Box-Behnken,a second order quadratic equation for extraction of naringin was established.The optimal extraction conditions for naringiin from Shatian pomelo white layer were:extraction solvent 52％ aqueous ethanol;microwave power 587 W;solid-liquid ratio 1∶20 (g∶ mL).Furthermore,the result indicated that appropriate microwave treatment can significantly influence antioxidant activities of extracts according to the results from DPPH,FRAP and ABTS.