一种金针菇水提液多糖含量的检测方法

摘要

以金针菇子实体为原料,运用热水浸提的方法提取金针菇多糖,采用苯酚硫酸法及3,5-二硝基水杨酸法分别测定水提液中的总糖及还原糖含量,以总糖和还原糖之差表示多糖含量,并进行方法学考查.结果表明,该方法样品处理简单,具有良好的适应性,其精密度、稳定性、加样回收率均符合要求,可用于金针菇水提液多糖含量的测定.%Using Flammulina velutipes fruiting body as raw material,the Flammulina velutipes polysaccharide is extracted by hot water.The contents of total sugar and reducing sugar in water extracts are determined by phenol sulfuric acid and 3,5-dinitrosalicylic acid (DNS) method.The concentration of palysaccharide depended on their concentration difference.The methodology is also investigated.The results show above methods are convenient and have good adaptability.The precision,stability and recovery rate of the sample are all in accordance with the requirements.The method can be used for the determination of polysaccharide in water extraction from Flammulina velutipes.