苹果果实中阿魏酸和儿茶素的毛细管电泳分离测定研究

摘要

Ferulic acid and catechin in apple made a qualitative and quantitative analysis by capillary electropho-resis. The best buffer to determine the separation of ferulic acid and catechin in apple is 30 mmol/L borax - boric acid +30% acetonitrile +50 mmol/L SDS, pH8, both of them are well separated within 10 minutes. The RSDs of getting the peak area and migration times are 2. 39% and 2. 92%, and 3. 41% and 5. 12% respectively. This method measured made the recovery of catechin in 90% to 102%, recovery of ferulic acid in 87% to 105%. This method can be used as a simple and good determination of apple fruits quality.%利用毛细管电泳法对苹果中阿魏酸和儿茶素做了定性定量分析.通过分离条件的优化,确定了分离苹果中阿魏酸和儿茶素的最佳缓冲液为30 mmol/L硼砂-硼酸+30％乙腈+50 mmol/L SDS,pH=8,这两种酚类物质在10 min内能较好地得到分离.得到儿茶素的峰面积和迁移时间的相对标准偏差(RSD)分别为2.39％和2.92％,阿魏酸的峰面积和迁移时间的RSD分别为3.41％和5.12％；儿茶素的回收率在90％～102％之间,阿魏酸的回收率在87％～105％之间.该方法简单、准确,可作为苹果果实品质测定的一种优良方法.