The full-length genomic sequence of foot-and-mouth disease virus(FMDV) Asia1/YNBS/58 strain was determined by RT-PCR and compared with other 17 reference strains.The results showed that the complete genome of Asial/YNBS/58 was 8164nt long including a 1061-nt 5′untranslated region(UTR),a 6990-nt open reading frame(ORF),and a 113-nt 3′UTR.The homology analysis indicated that the UTR regions and non-structural proteins were more conserved than the structural proteins in FMDV.VP1 exhibited the lowest conservation and VP4 was exceptionally conserved.The VP1-,VP2-,and VP3-based phylogenetic trees were divided into distinct clusters according to different serotypes,while the other gene-based phylogenetic trees exhibited some degree of intercross among serotypes.This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.
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