口蹄疫病毒OT株的全基因组序列测定及比较分析

摘要

参考GenBank上已发表的口蹄疫病毒(FMDV)全长基因组序列,设计了覆盖基因组全长的数对引物,通过RT-PCR方法对口蹄疫病毒OT株进行分段克隆及序列测定.结果表明不含poly(C)序列的OT株基因组全序列长8 142 nt,开放读码框(ORF)长6 969 nt、编码2 322 aa,5'UTR长1 004 nt,3'UTR长93 nt,3'UTR之后为23nt的poly(A)尾巴.应用分子生物学软件将OT株与FMDV其它参考毒株进行序列比对,并对其基因特征进行分析.结果显示,OT株的假结节(Pseudoknots)从第415-499位连续缺失85 nt,但是其3A基因却未发生碱基的缺失.其VP1基因的核苷酸同源性与O/Akesu58、OMⅢ两株最高,但OT株在进化时间上要比O/Akesu58、OMIII早很多,其毒株起源和遗传衍化关系还需要进一步的关注.%Several pairs of primers were designed to amplify the complete genome of foot-and-mouth disease virus (FMDV) OT strain by RT-PCR and the complete genome was sequenced. Then all regions of OT strain genome were compared with those of the reference strains which sequences were obtained from GenBank, and their genetic characteristic were analyzed by molecular biological software. As a result, the genome of FMDV OT strain was 8 142 nt without poly(C) and, and was composed of 5' untranslated region (UTR), open-reading frame (ORF) and 3' UTR, their nucleotide is 1 004 nt, 6 969 nt and 93 nt respectively. The pseudoknots area of OT strains loss 85 nt at 415-499 region, but its 3A gene were not loss. And the OT strain shared closest homology with O/Akesu58 and OM HI, but OT strain derived earlier than O/Akesu58 and Omfll strains for many years. The origin and genetic derivation relationship of OT strain still need further attention.