In this study, the whole genome of foot-and-mouth disease virus (FMDV) vaccine strain Asia1/JS/2005 in China was amplified and sequenced.The results showed that the complete genome is 8 174 nt, including a 1 092 nt 5’ untranslated region (UTR), a 6 990 nt open reading frame and a 92 nt 3’ UTR.By using the DNAstar software, homology analysis of genome sequences of strain Asia 1/JS/2005 and other 24 Asia1 strains were performed.The results indicated that non-structural proteins and 5’UTR were more conserved.The data revealed novel highly conserved and variable genomic regions, which was likely associated with biological function .Phylogenetic tree revealed that Asia1/JS/2005 is closely linked with Asia1/1/YZ/2006, Asia1/WHN/2006, Asia1/HN/2006 and Asia1/YS/2005, which suggested that they were genetically associated and Asia1/YS/05 was the most closely related strain with Asia1/JS/2005.%对中国口蹄疫病毒Asia1/JS/2005毒株的全基因组进行了分段扩增并测序。结果表明,该毒株的基因组全长为8174 nt,其中5’UTR为1092 nt;ORF为6990 nt;3’UTR为92 nt。利用DNAstar软件对该毒株和Asia1型其他24毒株基因组序列进行同源性分析表明,该毒株的非结构蛋白和5’UTR比较保守。研究还发现了可能与生物学功能相关的新的保守和变异区域。系统树分析表明Asia1/JS/2005, Asia1/1/YZ/2006, Asia1/WHN/2006,Asia1/HN/2006和Asia1/YS/2005属于同一分支,表明这些毒株间有着紧密的遗传关系,其中与其亲缘关系最为密切的是Asia1/YS/2005。
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机译:利用Asia 1型口蹄疫病毒VP1蛋白的单克隆抗体建立单抗竞争ELISA方法Establishment of Monoclonal Antibody Competitive ELISA Using Monoclonal Antibody Against VP1 Protein of Asia 1 Type Foot-and-Mouth Disease Virus