黄酒中挥发性和半挥发性成分检测固相微萃取条件的优化

摘要

A method for detection of volatile and semi-volatile compounds in rice wine by SPME-GC-MS was developed. The analysis parameters of extraction time, sample temperature, warm-up time, content of rice wine, content of sodium chloride, desorption time were optimized. The optimized method was as follows： 6 mL rice wine sample was put into 20 mL bottle and dissolved with 2.0 g NaCl. With 15 min warm-up time, the sample was extracted by 50/30 μm DVB/CAR/PDMS fiber for 30 min at the temperature of 60℃, then the volatiles and semi-volatiles were desorbed from the fiber at temperature of 250℃ for 6 min. It is a simple, quick methed, with less interference and high sensitivity.%采用固相微萃取-气相色谱联用质谱法分析黄酒中的挥发性和半挥发性成分,对萃取时间、萃取温度、预热时间、酒样添加量、盐添加量、解析时间等条件参数进行了优化,最终确定最佳条件为采用50/30μmDVB/CAR/PDMS萃取头,在20mL顶空瓶中,加入6mL黄酒样和2.0gNaCl,萃取温度60℃,预热时间15min,萃取时间30min,解析温度250℃,解析时间6min。本方法简单快速,干扰少,灵敏度高。