系统性红斑狼疮中Th17细胞相关细胞因子的变化

摘要

目的 探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)血浆中T辅助17(Th17)细胞相关的细胞因子白细胞介素(interleukin,IL)-17、IL-6、IL-23、IL-27和转化生长因子β(transforming growth factor-beta,TGF-β)水平的变化.方法 SLE组43例;健康时照组20例.采用酶联免疫吸附测定法测定血浆中IL-17、IL-6、IL-23、IL-27和TGF-β水平,分析它们的相关性.结果 SLE组与健康对照组比较,血浆中IL-17(74.32±22.76)ng/L vs(32.12±5.68)ng/L,IL-6(62.06±9.92)ng/L vs(37.98±15.41)ng/L,IL-23(30.29±12.59)ng/L vs(15.10±12.31)ng/L和IL-27(27.61±11.16)ng/L vs(16.17±8.35)ng/L的浓度都明显升高(P＜0.01),TGF-β的血浆浓度则明显降低(35.80±11.65)ng/L vs(69.08±28.76)ng/L(P＜0.01).IL-17与IL-6,IL-23和IL-27之间呈正相关(r=0.542、0.887、0.757,均P＜0.05),与TGF-β之间则呈负相关(r=-0.268,P＜0.05).结论 IL-17及其相关的细胞因子在SLE表达异常,IL-17在SLE发病中具有重要作用.%Objective To discuss the levels of T helper(Th)17-related cytokines interleukin(IL)-17, IL-6, IL-23, IL-27 and transforming growth factor-beta(TGF-β) in plasma of systemic lupus erythematosus(SLE). Methods The patients group included 43 patients of SLE with active renal disease and the control group included 20 healthy subjects. Plasma levels of IL-17, IL-6, IL-23, IL-27 and TGF-β were measured by enzyme-linked immunosorbent assay. And their correlations were analyzed. Results The levels of IL-17 (74. 32±22. 76) ng/L vs (32.12±5. 68) ng/L,IL-6(62.06± 9.92) ng/L vs (37. 98±15.41) ng/L, IL-23(30. 29±12. 59) ng/L vs (15.10±12. 31) ng/L and IL-27(27. 61±11. 16) ng/L vs (16.17±8. 35) ng/L were markly higher in SLE patients compared with healthy controls. The levels of TGFβ were significantly decreased in SLE patients than in healthy controls. Their difference was significant (35. 80±ll. 65) ng/L vs (69. 08±28. 76) ng/L( P <0.01). IL-17 level was correlated positively with the levels of IL-6, IL-23 and IL-27( r = 0. 542,0. 887,0. 757, all P <0. 05). IL-17 was correlated negatively with TGF-β( r =-0. 268, P <0. 05). Conclusion The expression of IL-17 and its related cytokines were abnormal in SLE, IL-17 played important role in the pathogenesis of SLE.