系统性红斑狼疮患者外周血Th17与Treg细胞及相关细胞因子表达的研究

摘要

Objective To investigate the population of T helper 17 cells and regulatory T cell in peripheral blood of system lupus erythematosus(SLE)patientsMethods Thirty-eigth patients with SLE were recruited,grouping accord-ing to SLE disease activity index score (SLEDAI).The percentages of Th1 7 and Treg cells were analysed by flow cyto-metric.Concentrations of Interleukin(IL)-17,IL-23,IL-6,transforming growth factor-βin plasma were used by enzyme linked immunosorbent assay(ELISA).Results The percent of Th17 and IL-17,IL-23 and IL-6 in SLE patients was sig-nificantly increased than that of normal controls(P<0.05),and it was different between the high active and inactive group(P<0.05).The population of Treg cells in the patients group was significantly lower than normal controls (P<0.05);Concentrations of TGF-βin SLE active group were lower than inactive group and controls(P<0.05).Conclusion The study suggests that Th1 7 and Treg cells may play an important role in the pathogenesis of SLE.%目的：检测系统性红斑狼疮（SLE）患者辅助性T细胞17（Th17）和调节性 T细胞（Treg）与其相关细胞因子在外周血中的表达。方法选择38例 SLE患者，根据病情严重程度 SLEDAI评分进行分组；使用流式细胞仪检测38例 SLE患者和对照组Th17和Treg细胞的数量；酶联免疫吸附法（ELISA）检测血浆中相关细胞因子的表达。结果 SLE患者外周血中Th17细胞及其相关细胞因子 IL-17、IL-23和 IL-6显著高于对照组，差异有统计学意义（P＜0．05），且疾病活动组的表达也显著高于非活动组（P＜0．05）；此外与对照组相比，SLE非活动组 Treg的表达降低（P＜0．05），并且疾病活动组的表达也低于非活动组（P＜0．05）；SLE活动组细胞因子 TGF-β的表达高于非活动组和对照组（P＜0．05）。结论 SLE患者外周血中Th17细胞及其相关细胞因子表达水平显著升高，而 Treg细胞的表达降低，两者平衡被打破，提示Th17／Treg的失衡可能在 SLE发病机制中发挥重要作用。