石墨炉原子吸收光谱分析法测定大鼠前列腺组织中的镉含量

摘要

目的 寻找可以消除大鼠前列腺组织中对镉检测产生干扰的基体改进剂,建立一种采用石墨炉原子吸收分光光度计准确测定大鼠前列腺组织中镉含量的新方法 .方法 取大鼠前列腺组织样本,用基体改进剂(1.0%HNO3+0.5%NH4H2PO4)进行稀释.使用Z-5000石墨炉原子吸收光谱仪全自动进样方法 进行测定.结果 在最适反应条件下,检测大鼠前列腺组织样本相对标准偏差(RSD)为0.12%～4.62%,回收率为95.8%～104.3%.结论 石墨炉原子吸收光谱分析法是一种理想的检测大鼠前列腺组织中镉含量的方法 ,具有良好的准确度和精密度.%Objective To develop an accurate method for determination of cadmium in prostate of the rats by the graphite furnace atomic absorption spectrometry(GFAAS) using suitable matrix modifer to eliminate the matrix interference in prostate of the rats. Methods After the sample was diluted by 1.0％ HNO3 + 0.5 ％ NH4H2PO4 ,the cadmium in prostate of the rats was deter mined by GFAAS. Results Under the optimum experimental conditions,the relative standard deviation of the method was 0. 12 ％ to 4.62％,and recovery was 95.8 ％ to 104.3％. Conclusion The method is good for determination of cadmium in prostate of the rats and accurate with good correction and precision.