葛根素对中波紫外线损伤HaCaT细胞的抗氧化作用

摘要

目的 研究葛根素不同处理方式对其抗氧化能力的影响.方法 以30 mJ/cm2中波紫外线(UVB)和1.0 mg/mL葛根素处理HaCaT细胞.实验分为阴性对照组、模型组、UVB照射前给药组、UVB照射后给药组和UVB照射前后给药组.UVB照射后24 h收集细胞,检测细胞内活性氧(ROS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPH-Px)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)和丙二醛(MDA).结果 UVB照射前给药处理和UVB照射前后给药处理,使细胞内T-AOC、CAT、GSH-Px升高,而ROS和MDA降低(P<0.05);UVB照射后给药处理可以升高SOD(P<0.05),但对其他指标无影响(P>0.05).结论 葛根素不同处理方式影响其抗氧化能力,其中预防性结合治疗性处理效果最佳,单纯预防性处理其次,单纯治疗性处理最差.%Objective To investigate the antioxidative effects of different treatments of puerarin. Methods HaCaT cells were treated by 30 mj/cm2 of UVB and 1. 0 mg/mL of puerarin, then were divided into 5 groups, including control group, modeling group, pre-treated, post-treated, pre-& post-treated by puerarin groups. Cells were harvested after 24 h of UVB irradiation. Reac-tive oxygen species(ROS) ,super oxidative dismutase(SOD) ,glutathione peroxidase(GSH-Px) ,catalase(CAT) ,total antioxidant ca-pacity(T-AOC) and malondialdehyde(MDA) were detected. Results On the contrary to ROS and MDA,the levels of T-AOC, CAT,GSH-Px were elevated in the pre-treated and the pre- & post-treated groups(P<0. 05). Except SOD increasement,the other indicators were showed no change in the post-treated group(P>0. 05). Conclusion Puerarin shows different antioxidative effects in the three kinds of treatments. Preventive and therapeutic treatment of puerarin exerts the best influence on antioxidation, whereas therapeutic treatment is the worst.